FastQC Report![[OK]](Icons/tick.png)
Basic Statistics
| Measure | Value |
|---|---|
| Filename | JRL21_R1_001.fastq |
| File type | Conventional base calls |
| Encoding | Sanger / Illumina 1.9 |
| Total Sequences | 32086628 |
| Filtered Sequences | 0 |
| Sequence length | 50 |
| %GC | 54 |
Per base sequence quality
Per sequence quality scores
![[FAIL]](Icons/error.png)
Per base sequence content
Per base GC content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
![[WARN]](Icons/warning.png)
Overrepresented sequences
| Sequence | Count | Percentage | Possible Source |
|---|---|---|---|
| TGGGACCTTCCACCCGGCCGGGGCCGACGAACCCCGAACCCCGAGCCGCG | 92842 | 0.2893479489337427 | No Hit |
| TGCGGACGCCGCCGGGTCTGCGCTTAGGGGGACGGAGGGCCCCCGGCGGG | 89387 | 0.27858022351242395 | No Hit |
| TGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAA | 86949 | 0.2709820427375541 | No Hit |
| TGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGA | 65617 | 0.20449951923898016 | No Hit |
| TAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCG | 62908 | 0.19605674987100544 | No Hit |
| TGGACGCCGCCGGGTCTGCGCTTAGGGGGACGGAGGGCCCCCGGCGGGCC | 54288 | 0.16919197617150672 | No Hit |
| TGGACCTTCCACCCGGCCGGGGCCGACGAACCCCGAACCCCGAGCCGCGC | 40856 | 0.12733030095901632 | No Hit |
| TGCCACCACCACCGTCGCTCGTGATTCTCGTCCATCCTCCGACCCGGTCC | 35199 | 0.109699903648336 | No Hit |
| TGGAAACACCTGCGCGCGGCCACCTCGAGCGTTCGCGTTCAGGGCGGGGG | 34431 | 0.10730638320735977 | No Hit |
Kmer Content
| Sequence | Count | Obs/Exp Overall | Obs/Exp Max | Max Obs/Exp Position |
|---|---|---|---|---|
| AAAAA | 4390555 | 4.954075 | 6.9162107 | 4 |
| TTTTT | 3550590 | 3.8867216 | 5.6042356 | 1 |
| GGAGG | 6679830 | 2.8944538 | 8.617663 | 2 |
| GGGAG | 6273190 | 2.7182517 | 9.831843 | 2 |
| GAGAG | 4603240 | 2.5338175 | 5.981804 | 3 |
| CACCA | 3106505 | 2.4148219 | 5.657779 | 33 |
| CCCCC | 3973565 | 2.4093878 | 5.05241 | 15 |
| TAAAA | 2114205 | 2.371145 | 5.9782953 | 3 |
| CCACC | 3427350 | 2.3530338 | 7.3111877 | 9 |
| GGGGA | 5315565 | 2.3033006 | 11.405604 | 2 |
| CTCCC | 3254330 | 2.2207477 | 5.483258 | 21 |
| TTTTC | 2235970 | 2.1748912 | 6.5412683 | 1 |
| CTTCA | 2370650 | 2.0613883 | 5.31088 | 7 |
| CTCCA | 2649925 | 2.047456 | 5.944891 | 7 |
| TTTAA | 1835540 | 2.0338123 | 8.947581 | 1 |
| TTTTA | 1820170 | 2.0045962 | 8.495324 | 1 |
| TTAAA | 1796895 | 2.003096 | 5.7970557 | 1 |
| TTCCA | 2280165 | 1.9827073 | 6.7142487 | 8 |
| GGGGG | 5773125 | 1.9692537 | 5.215899 | 2 |
| TGGAG | 3487075 | 1.9078355 | 28.1376 | 1 |
| TCCAG | 2745265 | 1.8905935 | 5.865212 | 8 |
| GAGGA | 3428990 | 1.8874607 | 5.11243 | 3 |
| TGCAG | 3071595 | 1.885431 | 17.067589 | 1 |
| TCCCC | 2760650 | 1.8838617 | 5.1258793 | 14 |
| CTTCC | 2420875 | 1.8591796 | 6.4076123 | 7 |
| TGGGG | 4314805 | 1.858362 | 25.266266 | 1 |
| GGAAG | 3375635 | 1.8580918 | 8.588407 | 2 |
| GAAGA | 2632105 | 1.8404557 | 5.3621836 | 3 |
| TATTT | 1648680 | 1.8157301 | 5.6545143 | 1 |
| TGCTG | 2966225 | 1.8097507 | 11.638475 | 1 |
| TGTTT | 2063020 | 1.7885779 | 22.787806 | 1 |
| CCCCG | 3277135 | 1.7711426 | 5.7094326 | 39 |
| TGCCA | 2569685 | 1.7696759 | 24.40092 | 1 |
| TGGGA | 3210550 | 1.7565442 | 30.33949 | 1 |
| CCTTC | 2256740 | 1.7331275 | 5.966025 | 6 |
| TGGAA | 2463275 | 1.7119969 | 24.521132 | 1 |
| ATATT | 1527145 | 1.6921047 | 6.719223 | 37 |
| TCCAC | 2180950 | 1.6851039 | 5.9502225 | 8 |
| TCTGC | 2415530 | 1.6534624 | 5.0411663 | 8 |
| GGAGA | 3001920 | 1.6523833 | 10.580484 | 2 |
| GGGAA | 2953070 | 1.6254942 | 8.916052 | 2 |
| GGGCA | 3280445 | 1.594781 | 7.5370464 | 2 |
| TGCTT | 2039810 | 1.5713859 | 13.821658 | 1 |
| TGGTG | 2856795 | 1.553555 | 6.254321 | 1 |
| GCCAG | 2810480 | 1.5329075 | 6.413899 | 2 |
| GCCAC | 2484495 | 1.5203398 | 7.122359 | 2 |
| TGAAG | 2170740 | 1.5086826 | 7.643801 | 1 |
| TGAAA | 1666195 | 1.4710466 | 6.229736 | 1 |
| GGAAA | 2096810 | 1.4661596 | 6.925229 | 2 |
| ACCCC | 2117210 | 1.4535621 | 5.3972135 | 38 |
| TTGAT | 1665995 | 1.4531492 | 5.3030686 | 40 |
| GAGAA | 2072010 | 1.4488186 | 5.564339 | 3 |
| TGGCA | 2345010 | 1.439433 | 12.377009 | 1 |
| GTTTT | 1630370 | 1.413483 | 6.169936 | 2 |
| ACCAT | 1610765 | 1.4091476 | 5.2588563 | 34 |
| GCACA | 2010260 | 1.3928303 | 5.6355352 | 2 |
| GCAGA | 2254365 | 1.3922046 | 5.6984506 | 2 |
| TGAGG | 2537795 | 1.388469 | 8.082746 | 1 |
| TGTCT | 1801275 | 1.3876283 | 8.776384 | 1 |
| TGCTC | 1960885 | 1.3422517 | 9.028757 | 1 |
| TGTCA | 1718640 | 1.332018 | 13.721001 | 1 |
| AACCT | 1507410 | 1.3187295 | 5.8262725 | 4 |
| TGCCT | 1921380 | 1.31521 | 8.060198 | 1 |
| CATAT | 1329060 | 1.3085197 | 5.6840067 | 36 |
| GGGAC | 2685445 | 1.3055232 | 6.3848033 | 2 |
| TGGGC | 2681410 | 1.295685 | 14.382419 | 1 |
| TGGTC | 2080905 | 1.2696002 | 5.477962 | 1 |
| TGTTC | 1646355 | 1.2682842 | 14.7235155 | 1 |
| TGCAA | 1600225 | 1.2477808 | 15.865781 | 1 |
| TGTTG | 1806385 | 1.2403274 | 9.509993 | 1 |
| GTTCA | 1593040 | 1.2346728 | 7.8079505 | 2 |
| TGAGA | 1774020 | 1.2329589 | 8.111848 | 1 |
| ATCCA | 1408975 | 1.2326156 | 5.0667 | 7 |
| ACCTT | 1394700 | 1.212755 | 5.6756706 | 5 |
| CCATA | 1384705 | 1.2113833 | 5.0528164 | 35 |
| GGACA | 1922485 | 1.187249 | 7.5196705 | 2 |
| TGGAT | 1696115 | 1.1716918 | 14.833607 | 1 |
| TGCCC | 1904765 | 1.1585423 | 7.8694444 | 1 |
| TGTAA | 1303025 | 1.1434609 | 8.833307 | 1 |
| TGTCC | 1664700 | 1.1395091 | 7.857575 | 1 |
| ATTGA | 1245925 | 1.093353 | 5.0884614 | 39 |
| TGCAC | 1570260 | 1.0813977 | 11.3932 | 1 |
| GGATG | 1972280 | 1.0790666 | 5.5968413 | 2 |
| TGCAT | 1381610 | 1.0708057 | 7.4450135 | 1 |
| TGGAC | 1696580 | 1.0414083 | 15.810094 | 1 |
| TAGAA | 1177040 | 1.0391824 | 5.5521183 | 1 |
| TGTGG | 1860735 | 1.0118872 | 6.362512 | 1 |
| TGGGT | 1824885 | 0.9923916 | 7.2925806 | 1 |
| TGTAG | 1427870 | 0.98638564 | 6.887236 | 1 |
| TGGTT | 1400555 | 0.9616703 | 5.746615 | 1 |
| GCAAC | 1387590 | 0.9614067 | 6.6211643 | 2 |
| TGAAC | 1212940 | 0.945794 | 5.1125383 | 1 |
| TGTGA | 1337375 | 0.92387086 | 6.3230314 | 1 |
| TGCCG | 1608505 | 0.8720187 | 11.586309 | 1 |
| TGTTA | 941080 | 0.8208485 | 7.2763214 | 1 |
| TGTAT | 929885 | 0.81108385 | 5.3947496 | 1 |
| GTTTG | 1165195 | 0.8000638 | 5.1597605 | 2 |
| TAGAG | 1149045 | 0.798596 | 6.1413527 | 1 |
| TGCGG | 1604890 | 0.77549946 | 12.722122 | 1 |
| GTTTA | 885290 | 0.7721862 | 6.287087 | 2 |
| TGCTA | 939965 | 0.7285123 | 6.615037 | 1 |
| TGTAC | 899895 | 0.69745636 | 5.537814 | 1 |
| TGCGA | 681730 | 0.41846502 | 5.9647336 | 1 |